In determining the lipase activities manually, emulsion with sera is incubated for 6 hours in the clinical laborstory.
An experiment had been carried out to see lipase activity increase by various activators under the best known, reaction condition
Preliminary check has been done by our experimental condition by using Monitrol Ⅱ. And allowable limits of error has come out of further tests.
As shown in Table 1 through 11 and Fig. 1, some chemicals have enzyme activating effects.
3 groups found in the experiments; are;
Inhibitors; _(n)-Octyl alcohol, NaF, NaCN…
No effectors; LiCO₃, NaNO₃, ZnSO₄…
Activators; CaCl₂, Desoxychollate, Bile, MgCl₂＆ NaCl…
It is sugsested the use of NaCl (68. 4mM) as a activator of a rapid lipase tests in the clinical laboratory.
|